Application
Western blot analysis of MLKL expression in Caki-1 whole cell lysate.Application
Western blot analysis of MLKL expression in mouse kidney tissue extract.WB
(A) Detection of RIP1, RIP3, MLKL, TNF-α, IL-6, IL-1β protein expression in each group.WB
(A) Electrophoresis of RIP1, RIP3, MLKL, TNF-α, IL-6, IL-1β protein expression in HT22 cells in each group.| Product Name | MLKL Antibody (N-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | MLKL (mixed lineage kinase domain-like) is a 471 amino acid protein that contains one protein kinase domain which is thought to be catalytically inactive. The gene encoding MLKL maps to chromosome 16 and is expressed as two isoforms which are produced by alternative splicing events. Chromosome 16, which is associated with a variety of genetic disorders, encodes over 900 genes and comprises nearly 3% of the human genome. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is associated with chromosome 16, as is Crohn's disease, which is a gastrointestinal inflammatory condition. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIPWB |
| WB:1:100~1:500 IP:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of MLKL of mouse and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of MLKL of mouse and human origin by WB, IP and ELISA |
| Entrez Gene | 197259 |
|---|
Application
Western blot analysis of MLKL expression in Caki-1 whole cell lysate.
Application
Western blot analysis of MLKL expression in mouse kidney tissue extract.
WB
(A) Detection of RIP1, RIP3, MLKL, TNF-α, IL-6, IL-1β protein expression in each group.
WB
(A) Electrophoresis of RIP1, RIP3, MLKL, TNF-α, IL-6, IL-1β protein expression in HT22 cells in each group.| Application Notes | WB:1:100~1:500 IP:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
