Application
Western blot analysis of Gβ expression in Y79 whole cell lysate.Application
Western blot analysis of Gβ 3 expression in non-transfected 293T: (A), mouse Gβ 3 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of Gβ 1 expression in non-transfected 293T: (A), mouse Gβ 1 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of Gβ expression in Jurkat whole cell lysate.Application
Western blot analysis of Gβ 2 expression in non-transfected: (A) and mouse Gβ 2 transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.Application
Western blot analysis of Gβ expression in bovine brain extract.| Product Name | Gβ Antibody (M-14) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within a conserved domain at the N-terminus of Gβ1, Gβ2, Gβ3 and Gβ4 of bovine origin |
| Modification Notes | Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (e.g., adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein å, ∫ and © polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-4). Most interest in G proteins has been focused on their å subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Evidence, however, has established an important regulatory role for the ∫© subunits (5-7). It is becoming increasingly clear that different G protein complexes expressed in different tissues carry structurally distinct members of the © as well as the å and ∫ subunits and that preferential associations between members of subunit families increase G protein functional diversity (8,9). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Gβ 1, Gβ 2, Gβ 3 and Gβ 4 of mouse, rat, human and bovine origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within a conserved domain at the N-terminus of G&beta 1 G&beta 2 G&beta 3 and G&beta 4 of bovine origin |
|---|---|
| Tissue Specificity | epitope mapping within a conserved domain at the N-terminus of Gβ1, Gβ2, Gβ3 and Gβ4 of bovine origin |
| Entrez Gene | 59345 |
|---|
Application
Western blot analysis of Gβ expression in Y79 whole cell lysate.
Application
Western blot analysis of Gβ 3 expression in non-transfected 293T: (A), mouse Gβ 3 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of Gβ 1 expression in non-transfected 293T: (A), mouse Gβ 1 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of Gβ expression in Jurkat whole cell lysate.
Application
Western blot analysis of Gβ 2 expression in non-transfected: (A) and mouse Gβ 2 transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).
Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.
Application
Western blot analysis of Gβ expression in bovine brain extract.| Application Notes | recommended for detection of Gβ 1, Gβ 2, Gβ 3 and Gβ 4 of mouse, rat, human and bovine origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
