Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of Cdc6 expression in HeLa whole cell lysate (A) and HeLa nuclear extract (B).Application
Western blot analysis of Cdc6 expression in HeLa (A) and NIH/3T3 (B) whole cell lysates and Jurkat (C) and HeLa (D) nuclear extracts.Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and Cdc6 siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with Cdc6 (H-304): .Application
Western blot analysis of Cdc6 expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic and nuclear staining.| Product Name | Cdc6 Antibody (H-304) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 257-560 of Cdc6 of human origin |
| Modification Notes | Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division control (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein Tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory Tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Cdc6 p62 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 257-560 of Cdc6 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 257-560 of Cdc6 of human origin |
| Entrez Gene | 990 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of Cdc6 expression in HeLa whole cell lysate (A) and HeLa nuclear extract (B).
Application
Western blot analysis of Cdc6 expression in HeLa (A) and NIH/3T3 (B) whole cell lysates and Jurkat (C) and HeLa (D) nuclear extracts.
Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and Cdc6 siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with Cdc6 (H-304): .
Application
Western blot analysis of Cdc6 expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic and nuclear staining.| Application Notes | recommended for detection of Cdc6 p62 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
