Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of gremlin expression in mouse brain tissue extract.| Product Name | gremlin Antibody (FL-184) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-184 representing full length gremlin 1 of human origin |
| Modification Notes | The gremlin protein family contains antagonists of bone morphogenetic protein (BMP) signaling that are expressed in the neural crest. All family members are secreted proteins that act as BMP antagonists in embryonic explants and are expressed in the proximal airway epithelium of the lung during embryonic development. Gremlin-1 is required for early limb outgrowth and patterning in the FGF4-SHH feedback loop, while gremlin-2 binds and blocks the activity of BMP-2 and BMP-4. The dorsaling factor gremlin belongs to a novel gene family that includes the head-inducing factor Cerberus and the tumor suppressor DAN. Additionally, secreted gremlin relays the sonic hedgehog signal from the polarizing region to the apical ectodermal ridge. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of gremlin-1 and gremlin-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-184 representing full length gremlin 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-184 representing full length gremlin 1 of human origin |
| Entrez Gene | 26585 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of gremlin expression in mouse brain tissue extract.| Application Notes | recommended for detection of gremlin-1 and gremlin-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
