Application
Western blot analysis of RBM28 expression in non-transfected: (A) and mouse RBM28 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RBM28 expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart tissue showing nuclear staining.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing nuclear staining.| Product Name | RBM28 Antibody (Z-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against RBM28 of human origin |
| Modification Notes | The RBM (RNA-binding motif) gene family encodes proteins with an RNA binding motif. RBM28 (RNA-binding motif protein 28) is a 759 amino acid protein that is suggested to be involved in ribosomal biogenesis. Localized to the nucleolus, the four RNA recognition motif (RRM) domain-containing RBM28 has been identified to interact with spliceosomal small nuclear RNAs (snRNAs). Mutations in the RRM3 domain of yeast NOP4 (a homolog of RBM28) lead to ribosomal depletion due to defective assembly of the 60S subunit, suggesting a functional role of RBM28 in the production of ribosomal machinery. A homozygous missense mutation in RBM28 is the cause of alopecia, neurological defects and endocrinopathy (ANE) syndrome, in which affected individuals suffer hair loss, severe mental retardation and central hypogonadotropic hypogonadism. The afflictions of this disease suggest that RBM28 is required for normal development of the hair follicle, the hypothalamic-hypophyseal axis and the nervous system. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of RBM28 of mouse, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against RBM28 of human origin |
|---|---|
| Tissue Specificity | raised against RBM28 of human origin |
| Entrez Gene | 55131 |
|---|
Application
Western blot analysis of RBM28 expression in non-transfected: (A) and mouse RBM28 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RBM28 expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart tissue showing nuclear staining.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing nuclear staining.| Application Notes | recommended for detection of RBM28 of mouse, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
