Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of SOCS-1 expression in HeLa (A), JAR (B) and JEG-3 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa + IFN-γ cells showing cytoplasmic localization.| Product Name | SOCS-1 Antibody (H-93) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 119-211 mapping within an internal region of SOCS-1 of human origin |
| Modification Notes | The SOCS (suppressor of cytokine signaling) gene family consists of a group of proteins that negatively regulate cytokine signal transduction (1). The SOCS family proteins contain a central SH2 domain and a carboxy-terminal region termed the “SOCS box.” The SOCS-1 (also called SSI-1 and JAB), SOCS-2 (also called SSI-2 and CIS2) and SOC-3 (also called SSI-3 and CIS3) genes are known to be upregulated by IL-6 and other cytokines (2). SOCS-4, SOCS-5, SOCS-6 and SOCS-7 were identified by their sequence homology with the SOCS box (3). CIS (for cytokine-inducible SH2-containing protein) is also a member of the SOCS family (4,5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of SOCS-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 119-211 mapping within an internal region of SOCS-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 119-211 mapping within an internal region of SOCS-1 of human origin |
| Entrez Gene | 8651 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of SOCS-1 expression in HeLa (A), JAR (B) and JEG-3 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa + IFN-γ cells showing cytoplasmic localization.| Application Notes | recommended for detection of SOCS-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
