| Product Name | IKAP Antibody (H-302) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1031-1332 mapping at the C-terminus of IKAP of human origin |
| Modification Notes | The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in the targeted degradation of IkB (1). IkB kinase å (IKKå), previously designated CHUK (2), interacts with IkB-å and specifically phosphorylates IkB-å on the sites that trigger its degradation, serines 32 and 36 (3). IKKå appears to be critical for NFkB activation in response to proinflammatory cytokines (4). Phosphorylation of the IkB by IKKå is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1 (5). The functional IKK complex contains three subunits, IKKå, IKKß and IKKg (also designated NEMO), and each appears to make essential contributions to IkB phosphorylation (6). IKAP (IKK-complex-associated protein) is a protein that acts as a scaffold, interacting with NIK, IKKå and IKKß and assembling them into an active kinase complex (7,8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IKAP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1031-1332 mapping at the C-terminus of IKAP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1031-1332 mapping at the C-terminus of IKAP of human origin |
| Entrez Gene | 8518 |
|---|
| Application Notes | recommended for detection of IKAP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
