Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.Application
Western blot analysis of human recombinant MAP LC3β fusion protein.| Product Name | MAP LC3β Antibody (H-50) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-50 mapping at the N-terminus of MAP LC3β of human origin |
| Modification Notes | Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3∫) and MAP-light chain 3 alpha (MAP-LC3å) are subunits of both MAP1A and MAP1B. MAP-LC3∫, a homolog of Apg8p, is essential for autophagy and associated to the autophagosome membranes after processing. Two forms of LC3∫, the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3∫, followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3’ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3∫ may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MAP LC3β and MAP LC3β2 of human origin, MAP LC3β of mouse and rat origin and, to a lesser extent, MAP LC3α of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-50 mapping at the N-terminus of MAP LC3&beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-50 mapping at the N-terminus of MAP LC3β of human origin |
| Entrez Gene | 81631 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.
Application
Western blot analysis of human recombinant MAP LC3β fusion protein.| Application Notes | recommended for detection of MAP LC3β and MAP LC3β2 of human origin, MAP LC3β of mouse and rat origin and, to a lesser extent, MAP LC3α of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
