Application
Western blot analysis of IL-2Rβ expression in Jurkat whole cell lysate.Application
Western blot analysis of IL-2Rβ expression in mouse PBL tissue extract.Application
Western blot analysis of IL-2Rβ expression in non-transfected: (A) and mouse IL-2Rβ transfected: (B) 293T whole cell lysates.Application
Western blot analysis of IL-2Rβ expression in non-transfected: (A) and human IL-2Rβ transfected: (B) 293T whole cell lysates.Application
Western blot analysis of IL-2Rβ expression in BJAB (A) and MOLT-4 (B) whole cell lysates.Application
Western blot analysis of IL-2Rβ expression in HuT 78 (A) and CCRF-CEM (B) whole cell lysates.Application
Antibodies tested include IL-2Rβ (C-20): (A,B), IL-2Rβ (M-20): (C,D) and IL-2Rβ (S-20): (E,F).Application
Immunofluorescence staining of methanol-fixed HuT 78 cells showing membrane staining.| Product Name | IL-2Rβ Antibody (M-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of IL-2Rβ of mouse origin |
| Modification Notes | The IL-2 receptor is a multicomponent complex consisting of three subunits, å, ∫ and ©, each of which is required for high affinity binding of IL-2. The a chain functions primarily in binding IL-2, whereas the ∫ and © chains contribute to IL-2 binding and are essential to IL-2-induced activation of signaling pathways leading to T cell growth. Both IL-4R and IL-7R were initially described as single chain high affinity ligand binding cytokine receptors. However, it is now well established that the IL-2R © chain functions as a second subunit of the high affinity IL-4R and IL-7R receptors. Consequently, the originally described subunits of these latter receptors are now referred to as IL-4Rå and IL-7Rå respectively, while the common subunit is referred to as ©c. Although the common © chain enhances ligand binding in these three cytokine receptors, it has no capacity to bind these ligands on its own. There is evidence that the gc chain is also a subunit of IL-13R. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IL-2Rβ of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of IL-2R&beta of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of IL-2Rβ of mouse origin |
| Entrez Gene | 16185 |
|---|
Application
Western blot analysis of IL-2Rβ expression in Jurkat whole cell lysate.
Application
Western blot analysis of IL-2Rβ expression in mouse PBL tissue extract.
Application
Western blot analysis of IL-2Rβ expression in non-transfected: (A) and mouse IL-2Rβ transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of IL-2Rβ expression in non-transfected: (A) and human IL-2Rβ transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of IL-2Rβ expression in BJAB (A) and MOLT-4 (B) whole cell lysates.
Application
Western blot analysis of IL-2Rβ expression in HuT 78 (A) and CCRF-CEM (B) whole cell lysates.
Application
Antibodies tested include IL-2Rβ (C-20): (A,B), IL-2Rβ (M-20): (C,D) and IL-2Rβ (S-20): (E,F).
Application
Immunofluorescence staining of methanol-fixed HuT 78 cells showing membrane staining.| Application Notes | recommended for detection of IL-2Rβ of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
