Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing membrane localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of IGF-IRα expression in non-transfected 293T: (A), human IGF-IRα transfected 293T: (B) and Hela (C) whole cell lysates.Application
Western blot analysis of IGF-IRα expression in HeLa whole cell lysates.Application
Western blot analysis of IGF-IRα expression in A-431 whole cell lysate (A) and rat placenta tissue extract (B).| Product Name | IGF-IRα Antibody (N-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of IGF-IRα of human origin |
| Modification Notes | Receptor tyrosine kinases (RTKs) are transmembrane molecular scaffolds that influence cellular processes including cell migration, metabolism, survival, proliferation and differentiation. Insulin-like growth factor-I receptor (IGF-IR) is an RTK that stimulates growth in many different cell types, blocks apoptosis, acts as an intermediate of many growth hormone responses and may stimulate the growth of some types of cancer. The IGF-IR cognate ligand insulin-like growth factor-I (IGF-I) promotes association of IGF-IR with Shc, GRB2 and Sos 1, which initiates Ras and ERK kinase cascades, thereby modifying transcription factor activity, such as activation of the Elk transcription factors. The modular phosphotyrosine binding (PTB) domains of insulin receptor substrate (IRS)-1 and -2 can associate with active IGF-IR and initiate phosphatidylinositol 3-kinase-dependent downstream signals. The human IGF-IR gene maps to chromosome 15q26.3 and encodes a 1,376 amino acid precursor protein that cleaves into a and b subunits. The human IGF-IIR gene maps to chromosome 6q26 and encodes a 2,491 amino acid transmembrane protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of IGF-IRα chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of IGF-IR&alpha of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of IGF-IRα of human origin |
| Entrez Gene | 3480 |
|---|
Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing membrane localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of IGF-IRα expression in non-transfected 293T: (A), human IGF-IRα transfected 293T: (B) and Hela (C) whole cell lysates.
Application
Western blot analysis of IGF-IRα expression in HeLa whole cell lysates.
Application
Western blot analysis of IGF-IRα expression in A-431 whole cell lysate (A) and rat placenta tissue extract (B).| Application Notes | recommended for detection of IGF-IRα chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
