Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human vagina tissue showing cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of MGC87895 expression in non-transfected 293T: (A), human MGC87895 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Ribosomal Protein S14 expression in LADMAC (A), MCP-5 (B), K-562 (C) and HeLa (D) whole cell lysates.Application
Western blot analysis of Ribosomal Protein S14 expression in HeLa whole cell lysate.Application
Western blot analysis of Ribosomal Protein S14 expression in non-transfected 293: (A), human Ribosomal Protein S14 transfected 293: (B) and K-562 (C) whole cell lysates.| Product Name | Ribosomal Protein S14 Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-130 mapping at the N-terminus of RPS14 of human origin |
| Modification Notes | Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein S14, also known as RPS14 or EMTB, is a 151 amino acid component of the small ribosomal 40S subunit. Localized to the cytoplasm, Ribosomal Protein S14 is a member of the S11P family of ribosomal proteins and is highly conserved among several species. Defects in the gene encoding Ribosomal Protein S14 may cause resistance to emetine, a protein synthesis inhibitor found in Chinese hamster ovary cells. Multiple isoforms of this protein exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ribosomal Protein S14 of mouse, rat and human origin by WB, IP, IF and ELISA also recommended for detection of MGC87895 of human origin and EG545121 of mouse origin also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-130 mapping at the N-terminus of RPS14 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-130 mapping at the N-terminus of RPS14 of human origin |
| Entrez Gene | 6208 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human vagina tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of MGC87895 expression in non-transfected 293T: (A), human MGC87895 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Ribosomal Protein S14 expression in LADMAC (A), MCP-5 (B), K-562 (C) and HeLa (D) whole cell lysates.
Application
Western blot analysis of Ribosomal Protein S14 expression in HeLa whole cell lysate.
Application
Western blot analysis of Ribosomal Protein S14 expression in non-transfected 293: (A), human Ribosomal Protein S14 transfected 293: (B) and K-562 (C) whole cell lysates.| Application Notes | recommended for detection of Ribosomal Protein S14 of mouse, rat and human origin by WB, IP, IF and ELISA also recommended for detection of MGC87895 of human origin and EG545121 of mouse origin also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
