Application
Western blot analysis of NOSIP expression in non-transfected 293T: (A), human NOSIP transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | NOSIP Antibody (FL-301) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-301 representing full length NOSIP of human origin |
| Modification Notes | Endothelial nitric oxide synthase (eNOS) interacting protein (NOSIP) is a modulator of eNOS activity. eNOS is an important nitric oxide (NO)-generating enzyme of the vasculature that is regulated by interactions with caveolin-1, Ca2+-calmodulin, HSP 90 and NOSIP. NOSIP modulates this activity by promoting the translocation of eNOS from the plasma membrane to intracellular sites, which in turn inhibits NO synthesis. NOSIP is involved in controlling airway and vascular diameter, synthesis of NO in ciliated epithelia and mucosal secretion, and is an important protein for mucociliary and bronchial function. NOSIP is highly expressed in endothelial cells and vascularized tissue. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NOSIP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-301 representing full length NOSIP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-301 representing full length NOSIP of human origin |
| Entrez Gene | 51070 |
|---|
Application
Western blot analysis of NOSIP expression in non-transfected 293T: (A), human NOSIP transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of NOSIP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
