Application
Western blot analysis of Myosin If expression in non-transfected 293T: (A), mouse Myosin If transfected 293T: (B) and HL-60 (C) whole cell lysates.Application
Western blot analysis of Myosin If expression in non-transfected: (A) and human Myosin If transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Myosin If expression in non-transfected: (A) and human Myosin If transfected: (B) 293T whole cell lysates.| Product Name | Myosin If Antibody (M-41) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1019-1059 mapping near the C-terminus of Myosin If of mouse origin |
| Modification Notes | Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Troponin facilitates interaction between Actin and Myosin by binding to Ca2+. Troponin is made up of at least two subunits, which are divergent in cardiac muscle, fast skeletal muscle and slow skeletal muscle. Myosin is a hexamer of 2 heavy chains (MHC) and 4 light chains (MLC) that interacts with Actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Myosin If (MYO1F), also designated Myosin-Ie, is considered an unconventional Myosin and is expressed in the cochlea. The MYO1F gene encoding for the 1098 amino acid protein maps to chromosome 19p13.3-13.2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Myosin If of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1019-1059 mapping near the C-terminus of Myosin If of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1019-1059 mapping near the C-terminus of Myosin If of mouse origin |
| Entrez Gene | 4542 |
|---|
Application
Western blot analysis of Myosin If expression in non-transfected 293T: (A), mouse Myosin If transfected 293T: (B) and HL-60 (C) whole cell lysates.
Application
Western blot analysis of Myosin If expression in non-transfected: (A) and human Myosin If transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Myosin If expression in non-transfected: (A) and human Myosin If transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Myosin If of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
