Application
Western blot analysis of EBF1 expression in non-transfected: (A) and human EBF1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of EBF1 expression in non-transfected: (A) and human EBF1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of EBF expression in human PBL whole cell lysate.Application
Western blot analysis of EBF expression in human PBL (A) and A549 (B) whole cell lysates.| Product Name | EBF Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of EBF of human origin |
| Modification Notes | B lymphocyte maturation is an intricate process that requires a distinct set of transcription factors with respect to the stage of cell differentiation and cell lineage. Among the transcriptional regulators involved in the early stages of B cell development, early B cell factor (EBF), also designated olfactory neuronal transcription factor 1 (OLF1), targets promoter elements for B lymphoid kinase (Blk) and genes encoding portions of the early stage B cell receptors (BCR), which are necessary for initiation of Ig light chain gene recombination and Src kinase (Blk) signaling. EBF is a basic helix-loop-helix (bHLH) homodimeric transcription factor composed of two subunits that interact with the core DNA sequence, CCCNNGGG, through a DNA recognition domain containing a zinc-coordination motif. Promoter elements to certain neuron-specific genes encoding olfactory-related proteins have been shown to contain EBF binding sites. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of EBF1-4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of EBF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of EBF of human origin |
| Entrez Gene | 1879 |
|---|
Application
Western blot analysis of EBF1 expression in non-transfected: (A) and human EBF1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of EBF1 expression in non-transfected: (A) and human EBF1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of EBF expression in human PBL whole cell lysate.
Application
Western blot analysis of EBF expression in human PBL (A) and A549 (B) whole cell lysates.| Application Notes | recommended for detection of EBF1-4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
