Application
Western blot analysis of Annexin IV expression in non-transfected 293T: (A), human Annexin IV transfected 293T: (B) and RAW 264.7 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human corpus uterine tissue showing cytoplasmic and membrane staining of glandular cells in low (A) and high (B) resolution. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Annexin IV expression in Caki-1 whole cell lysate.Application
Western blot analysis of Annexin IV expression in non-transfected 293T: (A), mouse Annexin IV transfected 293T: (B) and KNRK (C) whole cell lysates.Application
Western blot analysis of Annexin IV expression in non-transfected 293T: (A), human Annexin IV transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.| Product Name | Annexin IV Antibody (FL-319) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-319 representing full length Annexin IV of human origin |
| Modification Notes | The annexin family of calcium-binding proteins is composed of at least ten mammalian genes. It is characterized by a conserved core domain which binds to phospholipids in a Ca++-dependent manner and a unique amino terminal region which may confer binding specificity (1,2). Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. For example, the crystal structure of Annexin III has suggested a hydrophilic amino terminus with possible Ca++ channel activity (3). Similarly, Annexin V has ion channel properties (4). Annexin IV, also referred to as endonexin, functions to regulate Cl- flux by mediating calmodulin kinase II (CaMKII) activity and Annexin V has been shown to regulate PKC activity (5,6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Annexin IV and, to a lesser extent, Annexin V, VIII and XI of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-319 representing full length Annexin IV of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-319 representing full length Annexin IV of human origin |
| Entrez Gene | 307 |
|---|
Application
Western blot analysis of Annexin IV expression in non-transfected 293T: (A), human Annexin IV transfected 293T: (B) and RAW 264.7 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human corpus uterine tissue showing cytoplasmic and membrane staining of glandular cells in low (A) and high (B) resolution. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Annexin IV expression in Caki-1 whole cell lysate.
Application
Western blot analysis of Annexin IV expression in non-transfected 293T: (A), mouse Annexin IV transfected 293T: (B) and KNRK (C) whole cell lysates.
Application
Western blot analysis of Annexin IV expression in non-transfected 293T: (A), human Annexin IV transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.| Application Notes | recommended for detection of Annexin IV and, to a lesser extent, Annexin V, VIII and XI of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
