Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Immunopero-xidase staining of formalin fixed, paraffin-embedded human liver tissue showing membrane staining of hepatic sinusoids and Kupffer cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of DC-SIGNR expression in non-transfected: (A) and human DC-SIGNR transfected: (B) 293T whole cell lysates.| Product Name | DC-SIGN/DC-SIGNR Antibody (H-200) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 61-200 of DC-SIGN of human origin |
| Modification Notes | Dendritic cells (DC) are antigen-presenting immune system cells that are present on peripheral mucosal tissues and migrate to lymphoid tissues. DC-SIGN (DC-specific ICAM-3 grabbing nonintegrin) is a type II membrane protein that is exclusively expressed by DC. DC-SIGN, also designated CD209, binds to ICAM-3 to mediate the initial interaction between DC and resting T cells through the immunological synapse. The DC that are present in the initial sites of HIV-1 infection capture HIV-1 through DC-SIGN, which then facilitates the migration of DC to areas of T cell-rich secondary lymphoid organs, where it promotes efficient trans HIV-1 infection of these T cells. DC-SIGNR (DC-SIGN-related molecule), also designated CD209L and L-SIGN (liver/lymph node-specific ICAM-3 grabbing nonintegrin) is a type II integral membrane protein that is 77% identical to DC-SIGN. It is expressed on sinusoidal endothelial cells and binds the E2 glycoproteins of the hepatitis C virus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of DC-SIGN and DC-DIGNR of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 61-200 of DC-SIGN of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 61-200 of DC-SIGN of human origin |
| Entrez Gene | 30835 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Immunopero-xidase staining of formalin fixed, paraffin-embedded human liver tissue showing membrane staining of hepatic sinusoids and Kupffer cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of DC-SIGNR expression in non-transfected: (A) and human DC-SIGNR transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of DC-SIGN and DC-DIGNR of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
