Application
Western blot analysis of mouse recombinant IL-12B.| Product Name | IL-12B p40 Antibody (H-306) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 23-328 representing mature IL-12B p40 of human origin |
| Modification Notes | The interleukins (ILs) are a broad family of well characterized cytokines, primarily of hematopoietic cell origin. ILs are secreted by immune cells (mainly macrophages, B cells or T cells) that regulate a wide range of immune system functions. The functions of different ILs vary from regulating inflammatory and immune responses, functioning as an autocrine factor and regulating and/or inhibiting other ILs. IL-12 is responsible for the differentiation of naive CD4(+) T cells into type 1 helper T cells that produce interferon-© (IFN-©). It also activates production of tumor necrosis factor å (TNFå) from T and natural killer (NK) cells. IL-12 is a heterodimer composed of subunits IL-12A p35 and IL-12B p40. The p40 subunit of IL-12 also combines with p19, a protein that shows no biological activity by itself, to form a biologically active, composite cytokine, IL-23. IL-23 shares some in vivo functions with IL-12, including activation of the transcription factor Stat4 and IFN-© production and proliferation in PHA blast T cells, as well as in CD45RO (memory) T cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IL-12B p40 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 23-328 representing mature IL-12B p40 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 23-328 representing mature IL-12B p40 of human origin |
| Entrez Gene | 3593 |
|---|
Application
Western blot analysis of mouse recombinant IL-12B.| Application Notes | recommended for detection of IL-12B p40 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
