Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic and membrane staining of glandular cells.| Product Name | A33 Antibody (FL-319) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-319 representing full length A33 of human origin |
| Modification Notes | The A33 antigen is a transmembrane protein expressed almost exclusively in intestinal epithelium and in 95% of human colon cancers. Specifically, expression of the A33 protein occurs on the basolateral surfaces of intestinal epithelial cells of all lineages. The A33 antigen comprises an extracellular domain with two immunoglobulin-like domains, a single-span transmembrane domain, and a highly acidic cytoplasmic domain. Expression of A33 appears to be regulated by the intestine-specific homeobox transcription factor CDX1 and the gut-enriched Kruppel-like factor (GKLF). GKLF binds to the promoter region of the A33 gene in colon cancer cells, and mutations in the GKLF binding sequence leads to reduced expression of the A33 antigen. The therapeutic potential of administering the humanized monoclonal antibody A33 to colon cancer patients has proved encouraging. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of glycoprotein A33 of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-319 representing full length A33 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-319 representing full length A33 of human origin |
| Entrez Gene | 10223 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic and membrane staining of glandular cells.| Application Notes | recommended for detection of glycoprotein A33 of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
