Application
Western blot analysis of full length human recombinant IL-12A p35.Application
Western blot analysis of IL-12A p35 expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of IL-12A p35 expression in non-transfected: (A) and mouse IL-12A p35 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of IL-12A p35 expression in MOLT-4 whole cell lysate.| Product Name | IL-12A p35 Antibody (H-197) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 23-219 representing mature IL-12A p35 of human origin |
| Modification Notes | Interleukin-12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) and cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine that affects both natural killer (NK) cells and T lymphocytes. IL-12 is a heterodimer of subunit IL-12A p35 and subunit IL-12B p40 that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells. It is involved in mediating many aspects of antiviral immunity. In addition to stimulating T helper cell development, IL-12 is a potent inducer of interferon-© (IFN-©) production by T cells and NK cells. Increased IL-12 production is a consequence of EBV, CMV, LCMV or HSV infection. While it is unclear what separate effects can be attributed to each IL-12 subunit, evidence suggests IL-12A p35 is the biologically active component responsible for signal transduction via the IL-12 receptor. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IL-12A p35 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 23-219 representing mature IL-12A p35 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 23-219 representing mature IL-12A p35 of human origin |
| Entrez Gene | 3592 |
|---|
Application
Western blot analysis of full length human recombinant IL-12A p35.
Application
Western blot analysis of IL-12A p35 expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of IL-12A p35 expression in non-transfected: (A) and mouse IL-12A p35 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of IL-12A p35 expression in MOLT-4 whole cell lysate.| Application Notes | recommended for detection of IL-12A p35 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
