Application
Western blot analysis of Ajuba expression in non-transfected (A) and human Ajuba transfected (B) whole cell lysates.| Product Name | Ajuba Antibody (H-155) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 176-330 mapping within an internal region of Ajuba of human origin |
| Modification Notes | The LIM protein Ajuba (JUB), a member of the Zyxin family, mediates various cellular events. Ajuba is a component of the IL-1 signaling pathway modulating IL-1-induced NFkB activation by influencing the assembly and activity of the aPKC/p62/TRAF6 multiprotein signaling complex. Ajuba also plays a role in cadherin-mediated cell-cell adhesion and influences cell migration by regulating PI(4,5)P2 synthesis through direct activation of PIPKIa activity. Differentiating mouse embryonic stem cells show elevated Ajuba transcription. In adult mouse tissues, Ajuba is present in skin, brain and genitourinary organs. Immunofluorescence analysis of unsynchronized HeLa cells shows cytoplasmic staining. In cells synchronized at G2/M, Ajuba localizes to the centrosome, where it complexes with LATS2 to regulate the organization of the spindle apparatus through recruitment of g Tubulin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ajuba of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 176-330 mapping within an internal region of Ajuba of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 176-330 mapping within an internal region of Ajuba of human origin |
| Entrez Gene | 84962 |
|---|
Application
Western blot analysis of Ajuba expression in non-transfected (A) and human Ajuba transfected (B) whole cell lysates.| Application Notes | recommended for detection of Ajuba of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
