Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of GCK expression in non-transfected: (A) and human GCK transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GCK expression in non-transfected: (A) and mouse GCK transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GCK expression in rat pancreas extract.| Product Name | GCK Antibody (H-88) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 318-405 of GCK of human origin |
| Modification Notes | Glucokinase (also designated hexokinase IV, HXKIV or GCK) plays a key role in the regulation of glucose-induced insulin secretion. GCK is expressed in pancreatic beta cells where it functions as the major glucose sensor of the body, determining the “set point” for insulin secretion. GCK is also expressed in the liver, where it catalyzes the first committed step in the disposal of glucose. Phosphorylation of glucose by glucokinase appears to be the rate-limiting step for glucose catabolism. A lack of glucokinase activity leads to reduced insulin secretion and hyperglycemia, and has been implicated as a cause for maturity onset diabetes of the youth (MODY). In fact, heterozygous point mutations in the gene encoding GCK have been detected in individuals suffering from MODY. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GCK of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 318-405 of GCK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 318-405 of GCK of human origin |
| Entrez Gene | 2645 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of GCK expression in non-transfected: (A) and human GCK transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GCK expression in non-transfected: (A) and mouse GCK transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GCK expression in rat pancreas extract.| Application Notes | recommended for detection of GCK of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
