Application
Western blot analysis of GCN5 expression in non-transfected 293T: (A), human GCN5 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of GCN5 expression in non-transfected: (A) and human GCN5 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GCN5 expression in non-transfected: (A) and human GCN5 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GCN5 expression in non-transfected: (A) and human GCN5 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of 293 cells transfected with pEBB (A), pEBB/GCN5 (B), pCMV (C) or pCMV/PCAF (D). Kindly provided by Dr. Xicheng Mao, Burstein Lab, University of Michigan.Application
Western blot analysis of GCN5 expression in K-562 (A) and HeLa (B) nuclear extracts.| Product Name | GCN5 Antibody (H-75) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 376-450 mapping to an internal region of GCN5 of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPELISAIFIPWB |
| WB:1:100~1:500 IP:1:20~1:100 IF:1:5~1:20 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 376-450 mapping to an internal region of GCN5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 376-450 mapping to an internal region of GCN5 of human origin |
| Entrez Gene | 2648 |
|---|
Application
Western blot analysis of GCN5 expression in non-transfected 293T: (A), human GCN5 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of GCN5 expression in non-transfected: (A) and human GCN5 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GCN5 expression in non-transfected: (A) and human GCN5 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GCN5 expression in non-transfected: (A) and human GCN5 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of 293 cells transfected with pEBB (A), pEBB/GCN5 (B), pCMV (C) or pCMV/PCAF (D). Kindly provided by Dr. Xicheng Mao, Burstein Lab, University of Michigan.
Application
Western blot analysis of GCN5 expression in K-562 (A) and HeLa (B) nuclear extracts.| Application Notes | WB:1:100~1:500 IP:1:20~1:100 IF:1:5~1:20 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
