Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing membrane localization.Application
Western blot analysis of ANKRD50 expression in NIH/3T3 (A) and HEK293 (B) whole cell lysates.Application
Western blot analysis of ANKRD50 expression in non-transfected: (A) and human ANKRD50 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ANKRD50 expression in non-transfected: (A) and human ANKRD50 transfected: (B) 293T whole cell lysates and mouse heart tissue extract (C).| Product Name | ANKRD50 Antibody (G-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of ANKRD50 of human origin |
| Modification Notes | Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. Mutations of ankyrin genes lead to severe genetic diseases, such as fatal cardiac arrhythmias and hereditary spherocytosis. ANKRD50 (ankyrin repeat domain 50) is a 1,429 amino acid phosphoprotein that contains nineteen ANK repeats. Conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, fruit fly and mosquito, ANKRD50 is encoded by a gene that maps to human chromosome 4q28.1. Chromosome 4 represents approximately 6% of the human genome and contains nearly 900 genes. Notably, the Huntingtin gene, which encodes an expanded glutamine tract in cases of Huntington's disease, is located on chromosome 4. FGFR-3 is also encoded by a gene that maps to human chromosome 4 and has been associated with thanatophoric dwarfism, achondroplasia, Muenke syndrome and bladder cancer. Chromosome 4 is also linked to Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ANKRD50 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other ANKRD family members also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of ANKRD50 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of ANKRD50 of human origin |
| Entrez Gene | 57182 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing membrane localization.
Application
Western blot analysis of ANKRD50 expression in NIH/3T3 (A) and HEK293 (B) whole cell lysates.
Application
Western blot analysis of ANKRD50 expression in non-transfected: (A) and human ANKRD50 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ANKRD50 expression in non-transfected: (A) and human ANKRD50 transfected: (B) 293T whole cell lysates and mouse heart tissue extract (C).| Application Notes | recommended for detection of ANKRD50 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other ANKRD family members also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
