Application
Western blot analysis of Sg III expression in non-transfected: (A) and mouse Sg III transfected: (B) 293T whole cell lysates.| Product Name | Sg III Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Sg III of human origin |
| Modification Notes | Chromogranins (secretogranins) are acidic glycoproteins that localize within secretory granules of endocrine, neuroendocrine and neuronal tissue. Family members include chromogranin A, chromogranin B (secretogranin I) chromogranin C and secretogranin III (SCG3, Sg III). High levels of chromogranin A expression is a characteristic of neuro-endocrine tumors. Pancreastatin is a peptide derived from chromogranin A which inhibits insulin secretion, exocrine pancreatic secretion and gastric acid secretion. Sg III is an acidic secretory protein expressed in neuronal and endocrine cells. In the anterior lobe of the rat pituitary gland, Sg III is present in mammotropes and thyrotropes, moderately in gonadotropes and corticotropes, though not in somatotropes. Sg III and carboxypeptidase E (CPE) bind specifically to cholesterol-rich secretory granule (SG) membranes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Secretogranin III of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Sg III of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Sg III of human origin |
| Entrez Gene | 29106 |
|---|
Application
Western blot analysis of Sg III expression in non-transfected: (A) and mouse Sg III transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Secretogranin III of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
