| Product Name | ENDOG Antibody (H-152) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 145-297 mapping at the C-terminus of ENDOG of human origin |
| Modification Notes | Endonuclease G (ENDOG), a nuclear encoded protein, localizes to the mitochondria (1). This sugar-nonspecific nuclease, responsible for major mitochondrial nuclease activity, preferentially cleaves single-stranded DNA(ssDNA) (2). Synthesized as a propeptide with an amino-terminal presequence that targets the nuclease to mitochondria, ENDOG translocates to neuclei on apoptotic stimulation and act as a nuclease without sequence specificity (3,4). Both exonucleases and DNase I stimulate the ability of ENDOG to generate double-stranded DNA cleavage products at physiological ionic strengths, suggesting that these activities work in concert with ENDOG in apoptotic cells to ensure efficient DNA breakdown (5). In addition to deoxyribonuclease activities, ENDOG also has ribonuclease (RNase) and RNase H activities (3). ENDOG is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA (3). ENDOG exists in the mitochondrial intermembrane space, but not in the matrix where mtDNA replication occurs (4). This enzyme provides an important nicking function for mitochondrial DNA specifically cleaving DNA at GC tracts (1,2). Human ENDOG maps to chromosome 9q34.11 (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Endonuclease G of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 145-297 mapping at the C-terminus of ENDOG of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 145-297 mapping at the C-terminus of ENDOG of human origin |
| Entrez Gene | 2021 |
|---|
| Application Notes | recommended for detection of Endonuclease G of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
