Application
Western blot analysis of SNARK expression in non-transfected: (A) and human SNARK transfected: (B) 293T whole cell lysates.| Product Name | SNARK Antibody (H-128) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 501-628 mapping at the C-terminus of SNARK of human origin |
| Modification Notes | The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. SNARK (SNF1/AMP kinase-related kinase), also known as NUAK2 (NUAK family, SNF1-like kinase, 2), is s 628 amino acid protein that contains one protein kinase domain and belongs to the Ser/Thr protein kinase family. Using magnesium as a cofactor, SNARK catalyzes the ATP-dependent phosphorylation of target proteins and is involved in regulating cell tolerance to stress-induced glucose starvation. Additionally, SNARK is thought to induce cell-cell detachment and may protect cells from Fap-1-mediated apoptosis, possibly playing a role in the motility and invasiveness of tumor cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SNARK of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 501-628 mapping at the C-terminus of SNARK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 501-628 mapping at the C-terminus of SNARK of human origin |
| Entrez Gene | 81788 |
|---|
Application
Western blot analysis of SNARK expression in non-transfected: (A) and human SNARK transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of SNARK of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
