Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear staining of cells in tubules.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of HNF-1 expression in Hep G2 whole cell lysate.| Product Name | HNF-1 Antibody (H-205) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 80-284 mapping within an internal region of HNF-1α of human origin |
| Modification Notes | HNF-1 (å and ∫), HNF-3 (å, ∫ and ©), HNF-4 (å and ©), and HNF-6 compose, in part, a homoeprotein family designated the Hepatocyte Nuclear Factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF-3å, HNF-3∫ and HNF-3© regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3å and HNF-3∫ have also been shown to be involved in gastrulation events such as body axis formation. HNF-4å and HNF-4© have been shown to be important for early embryo development. HNF-4å is expressed in liver, kidney, pancreas, small intestine, testis and colon; and HNF-4© is expressed in each of these tissues except liver. HNF-6 has been shown to bind to the promoter of HNF-3∫, which indicates a potential role of HNF-6 in gut endoderm epithelial cell differentiation. Evidence suggests that HNF-6 may also be a transriptional activator for at least 22 other hepatocyte-enriched genes, including cytochrome P450 2C13 and å-1 antitrypsin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of HNF-1α and HNF-1β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 80-284 mapping within an internal region of HNF-1&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 80-284 mapping within an internal region of HNF-1α of human origin |
| Entrez Gene | 6927 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear staining of cells in tubules.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of HNF-1 expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of HNF-1α and HNF-1β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
