Application
Western blot analysis of Nodal expression in HeLa (A), THP-1 (B) and MIA PaCa-2 (C) whole cell lysates.| Product Name | Nodal Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 238-347 mapping at the C-terminus of Nodal of human origin |
| Modification Notes | The transforming growth factor ∫ (TGF∫) superfamily is composed of numerous growth and differentiation factors, including TGF∫1-3, Mullerian inhibiting substance (MIS), growth/differentiation factor (GDF) 1-9, bone morphogenic protein (BMP) 2-8, glial cell line-derived neurotrophic factor (GDNF), Inhibin å, ∫-A, ∫-B and ∫-C, Lefty and Nodal. Members of the TGF∫ superfamily are involved in embryonic development and adult tissue homeostasis. Ectodermal cells through the primitive streak delaminate and differentiate into mesoderm during gastrulation. Nodal expression is detectable in the primitive streak at the time of mesoderm formation, indicating a potential role for Nodal in mesoderm formation. Nodal has also been shown to be involved in the direction of heart looping and embryonic turning. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Nodal of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 238-347 mapping at the C-terminus of Nodal of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 238-347 mapping at the C-terminus of Nodal of human origin |
| Entrez Gene | 4838 |
|---|
Application
Western blot analysis of Nodal expression in HeLa (A), THP-1 (B) and MIA PaCa-2 (C) whole cell lysates.| Application Notes | recommended for detection of Nodal of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
