Application
Western blot analysis of GSS expression in non-transfected: (A) and human GSS transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GSS expression in CHO (A), SW480 (B), NIH/3T3 (C) and HT-1080 (D) whole cell lysates.Application
Western blot analysis of GSS expression in non-transfected: (A) and human GSS transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GSS expression in HeLa whole cell lysate.Application
Western blot analysis of GSS expression in non-transfected: (A) and mouse GSS transfected: (B) 293T whole cell lysates.| Product Name | GSS Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 81-380 mapping within an internal region of GSS of human origin |
| Modification Notes | GSS (Glutathione synthetase) is a 474 amino acid protein encoded by the gene located at human chromosome 20q11.2. GSS consists of three loops projecting from an antiparallel ∫-sheet, a parallel ∫-sheet and a lid of anti-parallel sheets, which provide access to the ATP-binding site. Although Southern blot and gene analysis suggest that GSS may be the only member of a unique family, the crystal structure indicates that GSS belongs to the ATP-GRASP superfamily. GSS is expressed in hemocytes and nucleated cells, including the brain. GSS occurs as a homodimer. There are two steps in the production of Glutathione, begining with ©-GCS and ending with GSS. In an ATP-dependent reaction, GSS produces Glutathione from ©-glutamylcysteine and glycine precursors. Partial hepatectomy, diethyl maleate, buthionine sulfoximine, tert-butylhaydroquinone and thioacetamide increase the ex-pression of GSS, which causes an increase in Glutathione levels. An inherited autosomal recessive disorder, 5-oxoprolinuria (pyroglutamic aciduria), is caused by GSS deficiencies, which leads to central nervous system damage, hemolytic anemia, metabolic acidosis and urinary excretion of 5-oxoproline. A missense mutation in the gene encoding GSS leads to a GSS deficiency restricted to erythrocytes, which causes only hemolytic anemia. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GSS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 81-380 mapping within an internal region of GSS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 81-380 mapping within an internal region of GSS of human origin |
| Entrez Gene | 2937 |
|---|
Application
Western blot analysis of GSS expression in non-transfected: (A) and human GSS transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GSS expression in CHO (A), SW480 (B), NIH/3T3 (C) and HT-1080 (D) whole cell lysates.
Application
Western blot analysis of GSS expression in non-transfected: (A) and human GSS transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GSS expression in HeLa whole cell lysate.
Application
Western blot analysis of GSS expression in non-transfected: (A) and mouse GSS transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of GSS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
