Application
Western blot analysis of ARHGAP30 expression in non-transfected: (A) and human ARHGAP30 transfected: (B) 293T whole cell lysates.| Product Name | ARHGAP30 Antibody (N-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the N-terminus of ARHGAP30 of human origin |
| Modification Notes | GTPase-activating proteins (GAPs) accelerate the intrinsic rate of GTP hydrolysis of Ras-related proteins, resulting in downregulation of their active form. ARHGAP30 (Rho GTPase activating protein 30), also known as Rho-type GTPase-activating protein 30, is a 1,101 amino acid protein that contains one Rho-GAP domain and exists as four alternatively spliced isoforms. Conserved in chimpanzee, dog, cow, mouse and rat, ARHGAP30 is among the most neurite-enriched GAPs, which also includes ARHGAP21, FNBP2 and Bcr). One of four single-nucleotide polymorphisms (SNPs) at the USF-1 gene locus, rs2774279, which is located in the promoter area of the USF-1 gene, which is in turn located within its flanking gene ARHGAP30, is linked to low-density lipoprotein cholesterol levels, incident type 2 diabetes mellitus and increased cardiovascular risk. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ARHGAP30 isoforms 1, 2 and 4 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other ARHGAP family members or ARHGAP30 isoform 3 also reactive with additional species, including bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the N-terminus of ARHGAP30 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the N-terminus of ARHGAP30 of human origin |
| Entrez Gene | 257106 |
|---|
Application
Western blot analysis of ARHGAP30 expression in non-transfected: (A) and human ARHGAP30 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of ARHGAP30 isoforms 1, 2 and 4 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other ARHGAP family members or ARHGAP30 isoform 3 also reactive with additional species, including bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
