Application
Western blot analysis of Ets-2 expression in non-transfected: (A) and mouse Ets-2 transfected: (B) 293T whole cell lysates and KNRK nuclear extract (C).Application
Western blot analysis of Ets-1/Ets-2 expression in Jurkat nuclear extract.Application
Western blot analysis of Erg-1/2/3 expression in non-transfected 293T: (A), human Erg-1/2/3 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of Ets-1 expression in non-transfected: (A) and mouse Ets-1 transfected: (B) 293T whole cell lysates and CCRF-CEM nuclear extract (C).Application
Western blot analysis of Ets-2 expression in non-transfected 293T: (A), mouse Ets-2 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
10% (A,C) and 1% (B,D) of input DNAs were amplified. Antibodies used included c-Jun (D): ; c-Fos (6-2H-2F): 7 and Ets-1/Ets-2 (C-275): . Data kindly provided by J. Imbert and reproduced with permission from Yeh, J.H., et al, Mol. Cell. Biol., 2001, 21:4515-4527.Application
Western blot analysis of Ets-1/Ets-2 expression in MM-142 (A), KNRK (B) and 3611-RF (C) nuclear extracts.| Product Name | Ets-1/Ets-2 Antibody (C-275) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within the highly conserved C-terminal domain of Ets-1/Ets-2 of human origin |
| Modification Notes | Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1–3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA-binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5’-GGAA-3’ element. Evidence indicates that the DNA binding activity by Ets-1 is regulated at the level of phosphorylation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISAGSChIP |
| recommended for detection of Ets-1 and Ets-2 of mouse, rat, human, Drosophila, Xenopus and C. elegans origin by WB, IP and ELISA may cross-react with FEV, Fli-1 and Erg-1/2/3 also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within the highly conserved C-terminal domain of Ets-1/Ets-2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within the highly conserved C-terminal domain of Ets-1/Ets-2 of human origin |
| Entrez Gene | 2113 |
|---|
Application
Western blot analysis of Ets-2 expression in non-transfected: (A) and mouse Ets-2 transfected: (B) 293T whole cell lysates and KNRK nuclear extract (C).
Application
Western blot analysis of Ets-1/Ets-2 expression in Jurkat nuclear extract.
Application
Western blot analysis of Erg-1/2/3 expression in non-transfected 293T: (A), human Erg-1/2/3 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of Ets-1 expression in non-transfected: (A) and mouse Ets-1 transfected: (B) 293T whole cell lysates and CCRF-CEM nuclear extract (C).
Application
Western blot analysis of Ets-2 expression in non-transfected 293T: (A), mouse Ets-2 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
10% (A,C) and 1% (B,D) of input DNAs were amplified. Antibodies used included c-Jun (D): ; c-Fos (6-2H-2F): 7 and Ets-1/Ets-2 (C-275): . Data kindly provided by J. Imbert and reproduced with permission from Yeh, J.H., et al, Mol. Cell. Biol., 2001, 21:4515-4527.
Application
Western blot analysis of Ets-1/Ets-2 expression in MM-142 (A), KNRK (B) and 3611-RF (C) nuclear extracts.| Application Notes | recommended for detection of Ets-1 and Ets-2 of mouse, rat, human, Drosophila, Xenopus and C. elegans origin by WB, IP and ELISA may cross-react with FEV, Fli-1 and Erg-1/2/3 also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
