Application
Western blot analysis of smoothelin expression in COLO 320DM whole cell lysate.| Product Name | smoothelin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of smoothelin of human origin |
| Modification Notes | The cytoskeletal protein smoothelin is highly conserved among vertebrates and is expressed exclusively by contractile smooth muscle cells where it localizes to the filament network. Smoothelin associates with actin stress fibers but does not interact with desmin. At least two isoforms of smoothelin are produced by alternative splicing. The short isoform lacks amino acids 1–544 at the amino-terminus of the long isoform. The short isoform is expressed in visceral muscle tissue, including intestine and stomach, but not in brain, while the long isoform is expressed in all vascularized organs. In the vascular system, smoothelin expression is limited to large veins and arteries capable of pulsatile contraction. As a marker for the highly differentiated contractile smooth muscle cell, smoothelin expression is useful for studying vascular malformation and injury. The gene encoding human smoothelin maps to chromosome 22q12.2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| recommended for detection of smoothelin long isoform of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: WB:1:100~1:200 IF:1:20~1:100 IP:1:20~1:100 ELISA:1:1000~5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of smoothelin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of smoothelin of human origin |
| Entrez Gene | 6525 |
|---|
Application
Western blot analysis of smoothelin expression in COLO 320DM whole cell lysate.| Application Notes | recommended for detection of smoothelin long isoform of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: WB:1:100~1:200 IF:1:20~1:100 IP:1:20~1:100 ELISA:1:1000~5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
