Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of Cdk9 expression in non-transfected: (A) and human Cdk9 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Cdk9 expression in non-transfected 293T: (A), human Cdk9 transfected 293T: (B) and HL-60 (C) whole cell lysates.Application
Western blot analysis of Cdk9 expression in non-transfected 293T: (A), mouse Cdk9 transfected 293T: (B) and HL-60 (C) whole cell lysates.Application
Western blot analysis of Cdk9 expression in non-transfected 293: (A), human Cdk9 transfected 293: (B) and HL-60 (C) whole cell lysates.Application
Western blot analysis of Cdk9 expression in non-transfected control (A) and Cdk9 siRNA transfected (B) HeLa cells. Blot probed with Cdk9 (C-20): . GAPDH (FL-335): used as specificity and loading control.Application
Western blot analysis of Cdk9 expression in HL-60 whole cell lysate.| Product Name | Cdk9 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Cdk9 of human origin |
| Modification Notes | A family of proteins designated cyclin dependent kinases (Cdks) are critical regulators of cell cycle progression. Cdk family members, including Cdc2 p34, Cdk1–9, PISSLRE, KKIALRE, PITSLRE and PCTAIRE 1–3 are constitutively expressed throughout the cell cycle. Cdc2 p34 activity peaks during mitosis and Cdk2 activity rises in late G1 or early S phase. Cdk4 and Cdk6 are critically involved in G1 to S phase progression. The functions of Cdk3, Cdk5b, PISSLRE, KKIALRE and PCTAIRE 1–3 are less well defined. Cdk9 (also designated PITALRE) has been shown to specifically phosphorylate the retinoblastoma protein. The more recently cloned Drosophila protein, P-TEFb, is thought to be the homolog of mammalian PITALRE. P-TEFb has been shown to be required for HIV Tat transcriptional activation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Cdk9 (also designated PITALRE) of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Cdk9 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Cdk9 of human origin |
| Entrez Gene | 1025 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of Cdk9 expression in non-transfected: (A) and human Cdk9 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Cdk9 expression in non-transfected 293T: (A), human Cdk9 transfected 293T: (B) and HL-60 (C) whole cell lysates.
Application
Western blot analysis of Cdk9 expression in non-transfected 293T: (A), mouse Cdk9 transfected 293T: (B) and HL-60 (C) whole cell lysates.
Application
Western blot analysis of Cdk9 expression in non-transfected 293: (A), human Cdk9 transfected 293: (B) and HL-60 (C) whole cell lysates.
Application
Western blot analysis of Cdk9 expression in non-transfected control (A) and Cdk9 siRNA transfected (B) HeLa cells. Blot probed with Cdk9 (C-20): . GAPDH (FL-335): used as specificity and loading control.
Application
Western blot analysis of Cdk9 expression in HL-60 whole cell lysate.| Application Notes | recommended for detection of Cdk9 (also designated PITALRE) of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
