Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic and nuclear staining of urothelial cells.Application
Western blot analysis of COMT expression in non-transfected 293T: (A), human COMT transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of COMT expression in non-transfected 293T: (A), human COMT transfected 293T: (B) and JAR (C) whole cell lysates.Application
Western blot analysis of COMT expression in non-transfected 293T: (A), human COMT transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of COMT expression in non-transfected 293T: (A), human COMT transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.Application
Western blot analysis of COMT expression in IMR-32 whole cell lysate.| Product Name | COMT Antibody (FL-271) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-271 representing full length Catechol-O-Methyltransferase of human origin |
| Modification Notes | Catechol-O-methyltransferase (COMT) plays a crucial role in the regulation of central dopaminergic systems by catalyzing the inactivation of catechol-amines. It is widely distributed in most tissues in soluble and membrane-bound forms. COMT-mediated methylation metabolism of catecholamine neurotransmitters is a first-line detoxification pathway. A Val158Met polymorphism of the COMT gene affects activity of the enzyme and influences performance and efficiency of the prefrontal cortex of the brain. Sequential conversion of estradiol to methoxyestradiol by COMT, contributes to the antimitogenic effects of estradiol on vascular smooth muscle cell growth via estrogen receptor-independent mechanisms. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of soluble and membrane bound COMT of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-271 representing full length Catechol-O-Methyltransferase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-271 representing full length Catechol-O-Methyltransferase of human origin |
| Entrez Gene | 1312 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic and nuclear staining of urothelial cells.
Application
Western blot analysis of COMT expression in non-transfected 293T: (A), human COMT transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of COMT expression in non-transfected 293T: (A), human COMT transfected 293T: (B) and JAR (C) whole cell lysates.
Application
Western blot analysis of COMT expression in non-transfected 293T: (A), human COMT transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of COMT expression in non-transfected 293T: (A), human COMT transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.
Application
Western blot analysis of COMT expression in IMR-32 whole cell lysate.| Application Notes | recommended for detection of soluble and membrane bound COMT of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
