Application
Western blot analysis of Six5 expression in K-562 (A) and Jurkat (B) whole cell lysates.| Product Name | Six5 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 440-739 mapping at the C-terminus of Six5 of human origin |
| Modification Notes | Six5 (homeobox protein SIX5), also known as SIX5, BOR2 or DMAHP (DM locus-associated homeodomain protein), is a transcription factor that is expressed in various structures of the adult eye. Localized to the cytoplasm in early development and to the nucleus in the later stages of development, Six5 is involved in regulation of organogenesis and in maintenance of retinal formation. Six5 is able to bind the 5'-TCA[AG][AG]TTNC-3' DNA sequence found in the myogenin and IGFBP5 promoters and, through this binding, can control transcription of the associated mRNA. Six5 is regulated via association with DACH1 (Dachshund homolog 1) and is coactivated by the EYA (eyes absent) proteins. Defects in the gene encoding Six5 are the cause of branchio-oto-renal syndrome type 2 (BOR2), an autosomal disorder characterized by hearing loss, a deep overbite and myopia. Two isoforms exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Six5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 440-739 mapping at the C-terminus of Six5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 440-739 mapping at the C-terminus of Six5 of human origin |
| Entrez Gene | 147912 |
|---|
Application
Western blot analysis of Six5 expression in K-562 (A) and Jurkat (B) whole cell lysates.| Application Notes | recommended for detection of Six5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
