Application
Western blot analysis of PTH2 Receptor expression in SK-N-MC whole cell lysate.| Product Name | PTH2 Receptor Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 25-84 mapping within an N-terminal extracellular domain of PTH2 Receptor of human origin |
| Modification Notes | Parathyroid hormone (PTH) and parathyroid hormone-related peptide (PTHrP) regulate calcium, phosphate and hydrogen ions in the kidney. In addition, PTHrP is also expressed in a variety of tissues, where it acts as an autocrine/ paracrine factor to influenceskeletal and cartilage development. Both ligands preferentially bind to the type 1 PTH/PTHrP receptor (PTH1R), whereas the type 2 PTH receptor (PTH2 receptor or PTH2R) binds only PTH, but not PTHrP. The PTH2 receptor also binds to tuberoinfundibular peptide of 39 residues (TIP39), which shares limited homology with PTH and may, subsequently, activate the PTH2 receptor through alternative methods. Both PTH receptors are members of the glucagon/secretin/calcitonin subfamily of G protein coupled receptors (GPCRs), and are characterized as seven transmembrane receptors that recruit G proteins and signal through intracellular adenyl cyclase/cAMP pathways. The PTH2 receptor is highly expressed in brain and pancreas, with lower expression in testis, and is thought to play a role in the regulation of several physiological systems, including pituitary hormone secretion and pain perception. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PTH2 Receptor of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 25-84 mapping within an N-terminal extracellular domain of PTH2 Receptor of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 25-84 mapping within an N-terminal extracellular domain of PTH2 Receptor of human origin |
| Entrez Gene | 5746 |
|---|
Application
Western blot analysis of PTH2 Receptor expression in SK-N-MC whole cell lysate.| Application Notes | recommended for detection of PTH2 Receptor of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
