Application
Western blot analysis of TRAP-α expression in HL-60 (A), Hep G2 (B) and HeLa (C) whole cell lysates.| Product Name | TRAP-α Antibody (FL-286) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-286 representing full length TRAP-α of human origin |
| Modification Notes | The TRAP proteins (translocon-associated proteins), TRAP-å, TRAP-∫, TRAP-© and TRAP-∂, are transmembrane proteins that comprise a heterotetramer complex (the signal sequence receptor (SSR) or TRAP complex) that localizes to the endoplasmic reticulum (ER) and functions in regulating the retention of ER resident proteins. The TRAP complex associates with the Sec61 translocon at the ER. Sec61 is the major complex mediating protein translocation across the ER membrane. In addition, the TRAP complex is involved in ER-associated degradation (ERAD); in response to ER stress the TRAP complex subunits are simultaneously induced by the XBP-1/IRE1å pathway. TRAP-å (also known as SSR1 or SSR-å), TRAP-∫ (also known as SSR-∫, SSR2 or TLAP) and TRAP-∂ (also known as SSR4) are all single-pass membrane proteins, while TRAP-© (also known as SSR3 or SSR-©) contains four transmembrane domains. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TRAP-α of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-286 representing full length TRAP-&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-286 representing full length TRAP-α of human origin |
| Entrez Gene | 6745 |
|---|
Application
Western blot analysis of TRAP-α expression in HL-60 (A), Hep G2 (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of TRAP-α of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
