Application
Western blot analysis of EphB3 expression in non-transfected 293T: (A), human EphB3 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of EphB1 expression in IMR-32 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing membrane and cytoplasmic localization.Application
Western blot analysis of EphB1 expression in mouse brain tissue extract.| Product Name | EphB1 Antibody (Q-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of EphB1 of rat origin |
| Modification Notes | The Eph subfamily represents the largest group of receptor protein tyrosine kinases identified to date (1–3). While the biological activities of these receptors have yet to be determined, there is increasing evidence that they are involved in central nervous system function and in development (1–3). The Eph subfamily receptors of human origin (and their murine/avian homologs) include EphA1 (Eph), EphA2 (Eck), EphA3 (Hek4), EphA4 (Hek8), EphA5 (Hek7), EphA6 (Hek12), EphA7 (Hek11/MDK1), EphA8 (Hek3), EphB1 (Hek6), EphB2 (Hek5), EphB3 (Cek10, Hek2), EphB4 (Htk), EphB5 (Hek9) and EphB6 (Mep). Ligands for Eph receptors include ephrin-A4 (LERK-4) which binds EphA3 and EphB1. In addition, ephrin-A2 (ELF-1) has been described as the ligand for EphA4, ephrin-A3 (Ehk1-L) as the ligand for EphA5 and ephrin-B2 (Htk-L) as the ligand for EphB4 (Htk) (4–7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of EphB1 and EphB3 of mouse, rat, human and avian origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of EphB1 of rat origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of EphB1 of rat origin |
| Entrez Gene | 2047 |
|---|
Application
Western blot analysis of EphB3 expression in non-transfected 293T: (A), human EphB3 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of EphB1 expression in IMR-32 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing membrane and cytoplasmic localization.
Application
Western blot analysis of EphB1 expression in mouse brain tissue extract.| Application Notes | recommended for detection of EphB1 and EphB3 of mouse, rat, human and avian origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
