Application
Western blot analysis of Endophilin B1 expression in NIH/3T3 (A), Hep G2 (B), HeLa (C) and RAW 264.7 (D) whole cell lysates.| Product Name | Endophilin B1 Antibody (M-62) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 250-311 mapping near the C-terminus of Endophilin B1 of mouse origin |
| Modification Notes | The Endophilins comprise a family of proteins that associate with Amphiphysin, Synaptojanin and Dynamin and are implicated in presynaptic vesicle trafficking at nerve terminals. The expression patterns of the Endophilins are consistent with their cellular functions at the neuronal synapse. Endophilin B1 is a member of the B subgroup of the Endophilin family that is required for maintenance of mitochondrial morphology and for the regulation of the outer mitochondrial membrane dynamics. The N-terminal domain of Endophilin B1 shares highest similarity with the lipid-binding and -modifying (LBM) domain of class A Endophilins. Endophilin B2 is also a member of the Endophilin B subroup that is ubiquitously expressed but shows highest levels in brain, adult lung, ovary, and spinal cord. Decreased levels of Endophilin B2 are found in Down Syndrome and may reflect brain dysgenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Endophilin B1 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 250-311 mapping near the C-terminus of Endophilin B1 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 250-311 mapping near the C-terminus of Endophilin B1 of mouse origin |
| Entrez Gene | 51100 |
|---|
Application
Western blot analysis of Endophilin B1 expression in NIH/3T3 (A), Hep G2 (B), HeLa (C) and RAW 264.7 (D) whole cell lysates.| Application Notes | recommended for detection of Endophilin B1 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
