Application
Western blot analysis of c-Maf expression in K-562 whole cell lysate.Application
Western blot analysis of MafB expression in non-transfected: (A) and human MafB transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing nuclear and cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of c-Maf expression in control (A) and c-Maf-transfected (B) COS cells.| Product Name | c-Maf Antibody (M-153) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 19-171 of c-Maf of mouse origin |
| Modification Notes | Members of the Maf family of basic region/leucine zipper (bZIP) transcription factors affect transcription in either a positive or negative fashion, depending on their particular protein partner and the context of the target promoter. c-Maf (Maf-2) and the closely related family members, neural retina leucine zipper (Nrl), L-Maf and Krml1/MafB (Maf-1), all bind to T-MARE sites and have been implicated in a wide variety of developmental and physiologic roles. The three small Maf family proteins (MafF, MafG and MafK) are components of NF-E2 that function as heterodimers with the large tissue-restricted subunit of NF-E2, called p45, and they are implicated in the transcriptional regulation of many erythroid-specific genes. MafB is expressed in a wide variety of tissues and encodes a protein containing a typical bZIP motif in its carboxy-terminal region. As a transcriptional activator, MafB plays a pivotal role in regulating lineage-specific gene expression during hematopoiesis by repressing Ets-1-mediated transcription of key erythroid-specific genes in myeloid cells. c-Maf interacts with the c-Myb DNA binding domain and forms Myb-Maf complexes, which, in turn, mediate the cooperative interactions between c-Myb and Ets-1 during early myeloid cell differentiation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of c-Maf and, to a lesser extent, MafA and MafB of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA may cross-react with Nrl: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 19-171 of c-Maf of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 19-171 of c-Maf of mouse origin |
| Entrez Gene | 9935 |
|---|
Application
Western blot analysis of c-Maf expression in K-562 whole cell lysate.
Application
Western blot analysis of MafB expression in non-transfected: (A) and human MafB transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing nuclear and cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of c-Maf expression in control (A) and c-Maf-transfected (B) COS cells.| Application Notes | recommended for detection of c-Maf and, to a lesser extent, MafA and MafB of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA may cross-react with Nrl: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
