Application
Western blot analysis of MyD88 expression in non-transfected: (A) and human MyD88 transfected: (B) CHO whole cell lysates.Application
Western blot analysis of MyD88 expression in human MyD88 transfected: (A) and non-transfected: (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of MyD88 expression in non-transfected control (A) and MyD88 siRNA transfected (B) J774A.1 cells. Blot probed with MyD88 (HFL-296): . GAPDH (FL-335): used as specificity and loading control.Application
Western blot analysis of MyD88 expression in J774A.1 (A), HEL 92.1.7 (B) and LNCaP (C) whole cell lysates.| Product Name | MyD88 Antibody (HFL-296) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-296 representing full length MyD88 of human origin |
| Modification Notes | Interleukin-1 (IL-1)-induced activation of the NF˚B pathway is mediated through the IL-1 receptor and the subsequent phosphorylation of IL-1 receptor-associated kinase (IRAK). The myeloid differentiation protein MyD88 was originally characterized as a protein upregulated in myeloleukemic cells following IL-6-induced growth arrest and terminal differentiation. MyD88 is now known to function as an adaptor protein for the association of IRAK with the IL-1 receptor. MyD88 is functionally homologous to the adaptor protein tube in the Toll signaling pathway of Drosophilia, and both proteins are members of the Toll/IL-1R superfamily. MyD88 contains a characteristic N-terminal death domain that is essential for NF˚B activation and an adjacent Toll/IL-1R homology domain (TIR domain). Collectively, these domains enable the protein-protein interactions of MyD88 with IRAK and the IL-1 receptor complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MyD88 of mouse, rat and human origin of WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-296 representing full length MyD88 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-296 representing full length MyD88 of human origin |
| Entrez Gene | 4615 |
|---|
Application
Western blot analysis of MyD88 expression in non-transfected: (A) and human MyD88 transfected: (B) CHO whole cell lysates.
Application
Western blot analysis of MyD88 expression in human MyD88 transfected: (A) and non-transfected: (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of MyD88 expression in non-transfected control (A) and MyD88 siRNA transfected (B) J774A.1 cells. Blot probed with MyD88 (HFL-296): . GAPDH (FL-335): used as specificity and loading control.
Application
Western blot analysis of MyD88 expression in J774A.1 (A), HEL 92.1.7 (B) and LNCaP (C) whole cell lysates.| Application Notes | recommended for detection of MyD88 of mouse, rat and human origin of WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
