Application
Western blot analysis of p16 expression in Saos-2 (A) and HeLa (B) cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of p16 (C-20): (A,B) and p16 (H-156): (C,D) for reactivity with human recombinant p16 (B,D) and control (A,C) fusion proteins.| Product Name | p16 Antibody (H-156) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-156 representing full length p16 of human origin |
| Modification Notes | The progression of cells through the cell cycle is regulated by a family of protein kinases known as cyclin-dependent kinases (Cdks). The sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. The cyclins function as differentially expressed positive regulators of Cdks. Negative regulators of the cycle include the p53-inducible protein p21 (also designated WAF1 or Cip1), Kip1 p27 and p16. The complexes formed by Cdk4 and the D-type cyclins have been strongly implicated in the control of cell proliferation during the G1 phase. It has been shown that p16 binds to Cdk4 and inhibits the catalytic activity of the Cdk4/cyclin D complex. Moreover, the gene encoding p16 exhibits a high frequency of homozygous deletions and point mutations in established human tumor cell lines. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of p16 and, to a lesser extent, p15 and p18 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-156 representing full length p16 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-156 representing full length p16 of human origin |
| Entrez Gene | 1029 |
|---|
Application
Western blot analysis of p16 expression in Saos-2 (A) and HeLa (B) cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of p16 (C-20): (A,B) and p16 (H-156): (C,D) for reactivity with human recombinant p16 (B,D) and control (A,C) fusion proteins.| Application Notes | recommended for detection of p16 and, to a lesser extent, p15 and p18 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
