Application
Western blot analysis of HA-probe expression in HA tagged β2B Tubulin (A), HA tagged 3pk (B) and HA tagged PTP 1B (C) transfected lysates.Application
Antibodies tested include HA-probe (Y-11)-G: (A) and HA-probe (Y-11): (B,C).Application
Immunofluorescence staining of methanol-fixed COS cells transfected with HA fusion protein showing cytoplasmic staining.| Product Name | HA-probe Antibody (Y-11) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of the influenza hemagglutinin (HA) protein |
| Modification Notes | Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. For example, the pCDM8 expression vector and derivatives thereof encode fusions between the target protein and an 11 amino acid peptide derived from the influenza protein hemagglutinin (HA). The HA epitope tag is useful in Western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-epitope tag. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISAChIP |
| recommended for detection of proteins containing the HA tag by WB, IP, IF, FCM and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of the influenza hemagglutinin (HA) protein |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of the influenza hemagglutinin (HA) protein |
Application
Western blot analysis of HA-probe expression in HA tagged β2B Tubulin (A), HA tagged 3pk (B) and HA tagged PTP 1B (C) transfected lysates.
Application
Antibodies tested include HA-probe (Y-11)-G: (A) and HA-probe (Y-11): (B,C).
Application
Immunofluorescence staining of methanol-fixed COS cells transfected with HA fusion protein showing cytoplasmic staining.| Application Notes | recommended for detection of proteins containing the HA tag by WB, IP, IF, FCM and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
