Application
Western blot analysis of GLDC expression in non-transfected 293T: (A), mouse GLDC transfected 293T: (B) and Hep G2 (C) whole cell lysates and mouse liver tissue extract (D).Application
Western blot analysis of GLDC expression in Hep G2 whole cell lysate.| Product Name | GLDC Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 256-555 mapping within an internal region of GLDC of human origin |
| Modification Notes | The glycine cleavage system is comprised of AMT (known as Protein T), GCSH (known as Protein H), DLD (known as Protein L) and GLDC (known as Protein P), all of which work together to catalyze the cleavage and degradation of glycine. GLDC (glycine dehydrogenase ), also known as GCE, GCSP (glycine cleavage system P protein) or HYGN1, is a 1,020 amino acid protein that localizes to the mitochondria and belongs to the gcvP family. GLDC binds to glycine and enables the methylamine group from glycine to be transferred to the Protein T. GLDC exists as a homodimer and utilizes pyridoxal phosphate as a cofactor. Mutations in the gene encoding GLDC leads to nonketotic hyperglycinemia (NKH), also known as glycine encephalopathy (GCE), an autosomal recessive disease characterized by accumulation of a large amount of glycine in body fluid and by severe neurological symptoms. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GLDC of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 256-555 mapping within an internal region of GLDC of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 256-555 mapping within an internal region of GLDC of human origin |
Application
Western blot analysis of GLDC expression in non-transfected 293T: (A), mouse GLDC transfected 293T: (B) and Hep G2 (C) whole cell lysates and mouse liver tissue extract (D).
Application
Western blot analysis of GLDC expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of GLDC of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
