Application
Western blot analysis of FR expression in JAR (A) and JEG-3 (B) whole cell lysates.Application
Western blot analysis of FR expression in JAR (A) and JEG-3 (B) whole cell lysates and mouse placenta tissue extract (C).| Product Name | FR Antibody (FL-257) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-257 representing full length Folate Receptor α of human origin |
| Modification Notes | Folate is an essential vitamin that must be obtained from food intake through intestinal absorption in mammals. Folate and reduced folic acid derivatives bind to the folate receptor (FR) family, which mediates the endocytosis of 5-methyltetrahydofolate into the cell. The folate receptors consist of five members, å, ∫, ©, ©’ (which is produced by alternative splicing) and ∂. å-FR and ∫-FR are attached to the membrane by a GPI anchor and are expressed in malignant tissues of epithelial and nonepithelial origin, respectively. ©-FR is expressed in tissues of hematopoietic origin, such as spleen, thymus and bone marrow, but the expression pattern of ∂-FR is elusive, which suggests that it is highly restricted both spatially and temporally. å-FR is used as a highly selective tumor marker and may be targeted for the delivery of therapeutic compounds to tumor cells by coupling to derivatives of folic acid. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of α, β, γ and, to a lesser extent, δ-FR of human origin and α and β, and, to a lesser extent, δ-FR of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-257 representing full length Folate Receptor &alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-257 representing full length Folate Receptor α of human origin |
| Entrez Gene | 2348 |
|---|
Application
Western blot analysis of FR expression in JAR (A) and JEG-3 (B) whole cell lysates.
Application
Western blot analysis of FR expression in JAR (A) and JEG-3 (B) whole cell lysates and mouse placenta tissue extract (C).| Application Notes | recommended for detection of α, β, γ and, to a lesser extent, δ-FR of human origin and α and β, and, to a lesser extent, δ-FR of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
