Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of β-TrCP/HOS expression in HeLa whole cell lysate.Application
Western blot analysis of β-TrCP expression in non-transfected 293T: (A), mouse β-TrCP transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of human recombinant β-TrCP fusion protein.| Product Name | β-TrCP/HOS Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 8-300 of β-TrCP of human origin |
| Modification Notes | b-tranducin repeats containing protein (b-TrCP), also designated E3RSIkB or FWD1, and HOS (homologous to Slimb) are F-box proteins that function as substrate recognition subunits of ubiquitin ligases. HOS and b-TrCP differ in their amino-terminal regions, but exhibit high homology within the F-box and WD40 repeat-containing regions. b-TrCP mediates ubiquitin/proteasome-dependent degradation of CD4 and ubiquitination of various proteins including IkB and b-catenin. HOS has also been shown to regulate the degradation of IkB and b-catenin in a similar manner. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of β-TrCP and HOS of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 8-300 of &beta -TrCP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 8-300 of β-TrCP of human origin |
| Entrez Gene | 8945 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of β-TrCP/HOS expression in HeLa whole cell lysate.
Application
Western blot analysis of β-TrCP expression in non-transfected 293T: (A), mouse β-TrCP transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of human recombinant β-TrCP fusion protein.| Application Notes | recommended for detection of β-TrCP and HOS of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
