Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing membrane and cytoplasmic staining of glandular cells.Application
Western blot analysis of Na+/K+-ATPase α3 expression in non-transfected 293: (A), human Na+/K+-ATPase α3 transfected 293: (B) and THP-1 (C) whole cell lysates.Application
Western blot analysis of Na+/K+-ATPase α2 expression in non-transfected 293T: (A), mouse Na+/K+-ATPase α2 transfected 293T: (B), HeLa (C) and NIH/3T3 (D) whole cell lysates.Application
Western blot analysis of Na+/K+-ATPase α1 expression in non-transfected 293T: (A), mouse Na+/K+-ATPase α1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Na+/K+-ATPase α1 expression in non-transfected 293T: (A), mouse NNa+/K+-ATPase α1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Na+/K+-ATPase α1 expression in non-transfected 293T: (A), human Na+/K+-ATPase α1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Na+/K+-ATPase α3 expression in Hep G2 whole cell lysate.Application
Western blot analysis of Na+/K+-ATPase α expression in 293T (A) and HeLa (B) whole cell lysates.Application
Immunofluorescence staining of normal mouse heart frozen section showing membrane staining.Application
Western blot analysis of Na+/K+-ATPase α expression in HeLa (A) and Caki-1 (B) whole cell lysates.| Product Name | Na+/K+-ATPase α Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 551-850 mapping within an internal region of Na+/K+-ATPase α1 of human origin |
| Modification Notes | The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as a oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, å, ∫ and ©, comprise the Na+/K+-ATPase oligomer. The å subunit contains the binding sites for ATP and the cations; the glycosylated ∫ subunit ensures correct folding and membrane insertion of the å subunits. The small © subunit co-localizes with the å subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The ∫ subunit, but not the © subunit, is essential for normal activity of Na+/K+-ATPase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Na+/K+-ATPase α1, 2 and 3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 551-850 mapping within an internal region of Na+/K+-ATPase &alpha 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 551-850 mapping within an internal region of Na+/K+-ATPase α1 of human origin |
| Entrez Gene | 476 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing membrane and cytoplasmic staining of glandular cells.
Application
Western blot analysis of Na+/K+-ATPase α3 expression in non-transfected 293: (A), human Na+/K+-ATPase α3 transfected 293: (B) and THP-1 (C) whole cell lysates.
Application
Western blot analysis of Na+/K+-ATPase α2 expression in non-transfected 293T: (A), mouse Na+/K+-ATPase α2 transfected 293T: (B), HeLa (C) and NIH/3T3 (D) whole cell lysates.
Application
Western blot analysis of Na+/K+-ATPase α1 expression in non-transfected 293T: (A), mouse Na+/K+-ATPase α1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Na+/K+-ATPase α1 expression in non-transfected 293T: (A), mouse NNa+/K+-ATPase α1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Na+/K+-ATPase α1 expression in non-transfected 293T: (A), human Na+/K+-ATPase α1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Na+/K+-ATPase α3 expression in Hep G2 whole cell lysate.
Application
Western blot analysis of Na+/K+-ATPase α expression in 293T (A) and HeLa (B) whole cell lysates.
Application
Immunofluorescence staining of normal mouse heart frozen section showing membrane staining.
Application
Western blot analysis of Na+/K+-ATPase α expression in HeLa (A) and Caki-1 (B) whole cell lysates.| Application Notes | recommended for detection of Na+/K+-ATPase α1, 2 and 3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
