Application
Western blot analysis of ARP-1 expression in non-transfected: (A) and mouse ARP-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ARP-1 expression in non-transfected: (A) and human ARP-1 transfected: (B) 293T whole cell lysates.| Product Name | COUP-TFI Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 261-320 mapping within an internal region of COUP-TFI of human origin |
| Modification Notes | COUP (chicken ovalbumin upstream promoter) transcription factors have been cloned in several species and identified as orphan members of the steroid/thyroid hormone receptor superfamily. COUP-TFI (also designated COUP or EAR-3) and ARP-1 (also designated COUP-TFII) exhibit highly regulated and overlapping expression in most tissues. COUP-TFs are highly expressed in the developing and central nervous system, suggesting that these factors may be important in neural development and differentiation. COUP-TFs can compete for binding to response elements which are common to other members of this family, including RAR, RXR, PPAR, HNF-4, VDR and TR. They have been shown to act as negative regulators as well as initiators of transcription. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of COUP-TFI, ARP-1, and, to a lesser extent, EAR-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 261-320 mapping within an internal region of COUP-TFI of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 261-320 mapping within an internal region of COUP-TFI of human origin |
| Entrez Gene | 7025 |
|---|
Application
Western blot analysis of ARP-1 expression in non-transfected: (A) and mouse ARP-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ARP-1 expression in non-transfected: (A) and human ARP-1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of COUP-TFI, ARP-1, and, to a lesser extent, EAR-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
