Application
Western blot analysis of AMAP-1 expression in non-transfected: (A) and mouse AMAP-1 transfected: (B) 293T whole cell lysates.| Product Name | AMAP-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 648-947 mapping at the C-terminus of AMAP-1 of human origin |
| Modification Notes | AMAP-1 (AMY-1-binding protein 1), also known as AMAM-1 or MYCBPAP (MYCBP associated protein), is a 947 amino acid protein that is expressed specifically in testis and is involved in spermatogenesis and synaptic processes. AMAP-1 colocalizes with MYCBP (AMY-1) in cytoplasm and also localizes to membrane. The gene encoding AMAP-1 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AMAP-1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 648-947 mapping at the C-terminus of AMAP-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 648-947 mapping at the C-terminus of AMAP-1 of human origin |
| Entrez Gene | 84073 |
|---|
Application
Western blot analysis of AMAP-1 expression in non-transfected: (A) and mouse AMAP-1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of AMAP-1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
