Application
Western blot analysis of LETM1 expression in HeLa (A), A549 (B), Jurkat (C), MOLT-4 (D) and ECV304 (E) whole cell lysates.| Product Name | LETM1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 440-739 mapping at the C-terminus of LETM1 of human origin |
| Modification Notes | LETM1 (Leucine zipper-EF-hand-containing transmembrane protein 1, mitochondrial) is a 739 amino acid protein that localizes to the mitochondrial membrane and contains one LETM1 domain and two EF-hand calcium-binding domains. Expressed in all fetal and adult tissues, LETM1 has a leucine zipper motif, a transmembrane domain and several phosphorylation sites and, via its EF-hand domains, may function as a calcium-binding protein. Additionally, LETM1 is thought to be involved in maintaining normal mitochondrial function and overall cell viability. Human LETM1 shares 84% similarity with its mouse counterpart, suggesting a conserved role between species. Deletions in the gene encoding LETM1 are associated with Wolf-Hirschhorn syndrome (WHS), a congenital syndrome characterized by a number of abnormalities, including mental retardation, seizures, heart defects, fused teeth, hearing loss, a webbed neck and renal abnormalities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of LETM1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 440-739 mapping at the C-terminus of LETM1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 440-739 mapping at the C-terminus of LETM1 of human origin |
Application
Western blot analysis of LETM1 expression in HeLa (A), A549 (B), Jurkat (C), MOLT-4 (D) and ECV304 (E) whole cell lysates.| Application Notes | recommended for detection of LETM1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
